LymphedemaV1, Main, Exploration, bibRecord, 012E48

Comparison of an enzyme linked immunosorbent assay (ELISA) and a radioallergosorbent test (RAST) for detection of IgE antibodies to Brugia malayi.

Identifieur interne : 012E48 ( Main/Exploration ); précédent : 012E47; suivant : 012E49

Comparison of an enzyme linked immunosorbent assay (ELISA) and a radioallergosorbent test (RAST) for detection of IgE antibodies to Brugia malayi.

Auteurs : Sitti Wahyuni [Indonésie] ; Ronald Van Ree ; Andarias Mangali ; Taniawati Supali ; Maria Yazdanbakhsh ; Erliyani Sartono

Source :

Parasite immunology [ 0141-9838 ]

RBID : pubmed:15053782

Descripteurs français

KwdFr :
- Adolescent, Adulte, Adulte d'âge moyen, Animaux, Anticorps antihelminthe (sang), Brugia malayi (immunologie), Enfant, Enfant d'âge préscolaire, Femelle, Filariose lymphatique (immunologie), Humains, Immunoglobuline E (sang), Indonésie, Mâle, Sujet âgé, Sujet âgé de 80 ans ou plus, Test ELISA (), Test RAST ().
MESH :
- immunologie : Brugia malayi, Filariose lymphatique.
- sang : Anticorps antihelminthe, Immunoglobuline E.
- Adolescent, Adulte, Adulte d'âge moyen, Animaux, Enfant, Enfant d'âge préscolaire, Femelle, Humains, Indonésie, Mâle, Sujet âgé, Sujet âgé de 80 ans ou plus, Test ELISA, Test RAST.
Wicri :
- geographic : Indonésie.

English descriptors

KwdEn :
- Adolescent, Adult, Aged, Aged, 80 and over, Animals, Antibodies, Helminth (blood), Brugia malayi (immunology), Child, Child, Preschool, Elephantiasis, Filarial (immunology), Enzyme-Linked Immunosorbent Assay (methods), Enzyme-Linked Immunosorbent Assay (statistics & numerical data), Female, Humans, Immunoglobulin E (blood), Indonesia, Male, Middle Aged, Radioallergosorbent Test (methods), Radioallergosorbent Test (statistics & numerical data).
MESH :
- chemical , blood : Antibodies, Helminth, Immunoglobulin E.
- geographic : Indonesia.
- immunology : Brugia malayi, Elephantiasis, Filarial.
- methods : Enzyme-Linked Immunosorbent Assay, Radioallergosorbent Test.
- statistics & numerical data : Enzyme-Linked Immunosorbent Assay, Radioallergosorbent Test.
- Adolescent, Adult, Aged, Aged, 80 and over, Animals, Child, Child, Preschool, Female, Humans, Male, Middle Aged.

Abstract

The enzyme linked immunosorbent assay (ELISA) for specific IgE antibodies to Brugia malayi was compared with the radioallergosorbent test (RAST) for use in immunoepidemiological studies of lymphatic filariasis. Sera used were from individuals (aged 5-82 years) living in an area endemic for lymphatic filariasis in South Sulawesi, Indonesia. The percentage of positive IgE ELISA reactions (52.6%) among the population was lower than the percentage of positive RAST (94.5%). Although an overall significant concordance was found between the two assays (P < 0.001), 328 (42.7%) individuals with a positive RAST result were negative in the ELISA, whereas only 6 (0.8%) subjects were positive by ELISA, yet negative by RAST. When the population was divided into those with active infection (positive for anti-filarial IgG4) and those not infected (mf-negative and negative for anti-filarial IgG4), the correlation between the two tests was higher in the IgG4-positive (rho = 0.70) than in the IgG4-negative (rho = 0.52) group. These results indicate that in assessment of B. malayi specific IgE antibody, RAST is superior to ELISA. However, given the use of radioactivity in the RAST method and given our results obtained in subjects with high anti-filarial IgG4, one could consider using the IgE-ELISA in areas with high endemicity for filariasis. In areas with low endemicity or where control programs are implemented, sera will have to be tested by RAST.

DOI: 10.1111/j.0141-9838.2004.00673.x
PubMed: 15053782

Affiliations:

Indonésie

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Comparison of an enzyme linked immunosorbent assay (ELISA) and a radioallergosorbent test (RAST) for detection of IgE antibodies to Brugia malayi.</title>
<author><name sortKey="Wahyuni, Sitti" sort="Wahyuni, Sitti" uniqKey="Wahyuni S" first="Sitti" last="Wahyuni">Sitti Wahyuni</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Parasitology, Hasanuddin University, Makassar, Indonesia.</nlm:affiliation>
<country xml:lang="fr">Indonésie</country>
<wicri:regionArea>Department of Parasitology, Hasanuddin University, Makassar</wicri:regionArea>
<wicri:noRegion>Makassar</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Van Ree, Ronald" sort="Van Ree, Ronald" uniqKey="Van Ree R" first="Ronald" last="Van Ree">Ronald Van Ree</name>
</author>
<author><name sortKey="Mangali, Andarias" sort="Mangali, Andarias" uniqKey="Mangali A" first="Andarias" last="Mangali">Andarias Mangali</name>
</author>
<author><name sortKey="Supali, Taniawati" sort="Supali, Taniawati" uniqKey="Supali T" first="Taniawati" last="Supali">Taniawati Supali</name>
</author>
<author><name sortKey="Yazdanbakhsh, Maria" sort="Yazdanbakhsh, Maria" uniqKey="Yazdanbakhsh M" first="Maria" last="Yazdanbakhsh">Maria Yazdanbakhsh</name>
</author>
<author><name sortKey="Sartono, Erliyani" sort="Sartono, Erliyani" uniqKey="Sartono E" first="Erliyani" last="Sartono">Erliyani Sartono</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="????"><PubDate><MedlineDate>2003 Nov-Dec</MedlineDate>
</PubDate>
</date>
<idno type="RBID">pubmed:15053782</idno>
<idno type="pmid">15053782</idno>
<idno type="doi">10.1111/j.0141-9838.2004.00673.x</idno>
<idno type="wicri:Area/PubMed/Corpus">004036</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">004036</idno>
<idno type="wicri:Area/PubMed/Curation">004036</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">004036</idno>
<idno type="wicri:Area/PubMed/Checkpoint">004036</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">004036</idno>
<idno type="wicri:Area/Ncbi/Merge">001954</idno>
<idno type="wicri:Area/Ncbi/Curation">001954</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">001954</idno>
<idno type="wicri:doubleKey">0141-9838::Wahyuni S:comparison:of:an</idno>
<idno type="wicri:Area/Main/Merge">014067</idno>
<idno type="wicri:Area/Main/Curation">012E48</idno>
<idno type="wicri:Area/Main/Exploration">012E48</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Comparison of an enzyme linked immunosorbent assay (ELISA) and a radioallergosorbent test (RAST) for detection of IgE antibodies to Brugia malayi.</title>
<author><name sortKey="Wahyuni, Sitti" sort="Wahyuni, Sitti" uniqKey="Wahyuni S" first="Sitti" last="Wahyuni">Sitti Wahyuni</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Parasitology, Hasanuddin University, Makassar, Indonesia.</nlm:affiliation>
<country xml:lang="fr">Indonésie</country>
<wicri:regionArea>Department of Parasitology, Hasanuddin University, Makassar</wicri:regionArea>
<wicri:noRegion>Makassar</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Van Ree, Ronald" sort="Van Ree, Ronald" uniqKey="Van Ree R" first="Ronald" last="Van Ree">Ronald Van Ree</name>
</author>
<author><name sortKey="Mangali, Andarias" sort="Mangali, Andarias" uniqKey="Mangali A" first="Andarias" last="Mangali">Andarias Mangali</name>
</author>
<author><name sortKey="Supali, Taniawati" sort="Supali, Taniawati" uniqKey="Supali T" first="Taniawati" last="Supali">Taniawati Supali</name>
</author>
<author><name sortKey="Yazdanbakhsh, Maria" sort="Yazdanbakhsh, Maria" uniqKey="Yazdanbakhsh M" first="Maria" last="Yazdanbakhsh">Maria Yazdanbakhsh</name>
</author>
<author><name sortKey="Sartono, Erliyani" sort="Sartono, Erliyani" uniqKey="Sartono E" first="Erliyani" last="Sartono">Erliyani Sartono</name>
</author>
</analytic>
<series><title level="j">Parasite immunology</title>
<idno type="ISSN">0141-9838</idno>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term>
<term>Adult</term>
<term>Aged</term>
<term>Aged, 80 and over</term>
<term>Animals</term>
<term>Antibodies, Helminth (blood)</term>
<term>Brugia malayi (immunology)</term>
<term>Child</term>
<term>Child, Preschool</term>
<term>Elephantiasis, Filarial (immunology)</term>
<term>Enzyme-Linked Immunosorbent Assay (methods)</term>
<term>Enzyme-Linked Immunosorbent Assay (statistics & numerical data)</term>
<term>Female</term>
<term>Humans</term>
<term>Immunoglobulin E (blood)</term>
<term>Indonesia</term>
<term>Male</term>
<term>Middle Aged</term>
<term>Radioallergosorbent Test (methods)</term>
<term>Radioallergosorbent Test (statistics & numerical data)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Adolescent</term>
<term>Adulte</term>
<term>Adulte d'âge moyen</term>
<term>Animaux</term>
<term>Anticorps antihelminthe (sang)</term>
<term>Brugia malayi (immunologie)</term>
<term>Enfant</term>
<term>Enfant d'âge préscolaire</term>
<term>Femelle</term>
<term>Filariose lymphatique (immunologie)</term>
<term>Humains</term>
<term>Immunoglobuline E (sang)</term>
<term>Indonésie</term>
<term>Mâle</term>
<term>Sujet âgé</term>
<term>Sujet âgé de 80 ans ou plus</term>
<term>Test ELISA ()</term>
<term>Test RAST ()</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Helminth</term>
<term>Immunoglobulin E</term>
</keywords>
<keywords scheme="MESH" type="geographic" xml:lang="en"><term>Indonesia</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Brugia malayi</term>
<term>Filariose lymphatique</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Brugia malayi</term>
<term>Elephantiasis, Filarial</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term>
<term>Radioallergosorbent Test</term>
</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antihelminthe</term>
<term>Immunoglobuline E</term>
</keywords>
<keywords scheme="MESH" qualifier="statistics & numerical data" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term>
<term>Radioallergosorbent Test</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Adolescent</term>
<term>Adult</term>
<term>Aged</term>
<term>Aged, 80 and over</term>
<term>Animals</term>
<term>Child</term>
<term>Child, Preschool</term>
<term>Female</term>
<term>Humans</term>
<term>Male</term>
<term>Middle Aged</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Adolescent</term>
<term>Adulte</term>
<term>Adulte d'âge moyen</term>
<term>Animaux</term>
<term>Enfant</term>
<term>Enfant d'âge préscolaire</term>
<term>Femelle</term>
<term>Humains</term>
<term>Indonésie</term>
<term>Mâle</term>
<term>Sujet âgé</term>
<term>Sujet âgé de 80 ans ou plus</term>
<term>Test ELISA</term>
<term>Test RAST</term>
</keywords>
<keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Indonésie</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The enzyme linked immunosorbent assay (ELISA) for specific IgE antibodies to Brugia malayi was compared with the radioallergosorbent test (RAST) for use in immunoepidemiological studies of lymphatic filariasis. Sera used were from individuals (aged 5-82 years) living in an area endemic for lymphatic filariasis in South Sulawesi, Indonesia. The percentage of positive IgE ELISA reactions (52.6%) among the population was lower than the percentage of positive RAST (94.5%). Although an overall significant concordance was found between the two assays (P < 0.001), 328 (42.7%) individuals with a positive RAST result were negative in the ELISA, whereas only 6 (0.8%) subjects were positive by ELISA, yet negative by RAST. When the population was divided into those with active infection (positive for anti-filarial IgG4) and those not infected (mf-negative and negative for anti-filarial IgG4), the correlation between the two tests was higher in the IgG4-positive (rho = 0.70) than in the IgG4-negative (rho = 0.52) group. These results indicate that in assessment of B. malayi specific IgE antibody, RAST is superior to ELISA. However, given the use of radioactivity in the RAST method and given our results obtained in subjects with high anti-filarial IgG4, one could consider using the IgE-ELISA in areas with high endemicity for filariasis. In areas with low endemicity or where control programs are implemented, sera will have to be tested by RAST.</div>
</front>
</TEI>
<affiliations><list><country><li>Indonésie</li>
</country>
</list>
<tree><noCountry><name sortKey="Mangali, Andarias" sort="Mangali, Andarias" uniqKey="Mangali A" first="Andarias" last="Mangali">Andarias Mangali</name>
<name sortKey="Sartono, Erliyani" sort="Sartono, Erliyani" uniqKey="Sartono E" first="Erliyani" last="Sartono">Erliyani Sartono</name>
<name sortKey="Supali, Taniawati" sort="Supali, Taniawati" uniqKey="Supali T" first="Taniawati" last="Supali">Taniawati Supali</name>
<name sortKey="Van Ree, Ronald" sort="Van Ree, Ronald" uniqKey="Van Ree R" first="Ronald" last="Van Ree">Ronald Van Ree</name>
<name sortKey="Yazdanbakhsh, Maria" sort="Yazdanbakhsh, Maria" uniqKey="Yazdanbakhsh M" first="Maria" last="Yazdanbakhsh">Maria Yazdanbakhsh</name>
</noCountry>
<country name="Indonésie"><noRegion><name sortKey="Wahyuni, Sitti" sort="Wahyuni, Sitti" uniqKey="Wahyuni S" first="Sitti" last="Wahyuni">Sitti Wahyuni</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 012E48 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 012E48 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Sante
   |area=    LymphedemaV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:15053782
   |texte=   Comparison of an enzyme linked immunosorbent assay (ELISA) and a radioallergosorbent test (RAST) for detection of IgE antibodies to Brugia malayi.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:15053782" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a LymphedemaV1

This area was generated with Dilib version V0.6.31.
Data generation: Sat Nov 4 17:40:35 2017. Site generation: Tue Feb 13 16:42:16 2024

Serveur d'exploration sur le lymphœdème

Comparison of an enzyme linked immunosorbent assay (ELISA) and a radioallergosorbent test (RAST) for detection of IgE antibodies to Brugia malayi.

Comparison of an enzyme linked immunosorbent assay (ELISA) and a radioallergosorbent test (RAST) for detection of IgE antibodies to Brugia malayi.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur le lymphœdème
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.